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Robert Murray

I am sure that vectors have to be safe (experience in the virus field underlines this) and one should be a bit cautious. I am sure I would be unhappy following an incautious dose of LPS along with minicells of Gram negative bacteria. I note that there is at least one paper that had this in mind and used protoplasted, achromosomal bacterial minicells for DNA transfer purposes.( M.J. Giacolone et al., Cell. Microbiol. 8:1624, 2006) They recognised this as one of a number of necessary steps before there is utility. On the other hand maybe a tiny dose of some such stimulus primes useful processes.

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