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Mark O. Martin

My former thesis advisor, Sharon Long, told a great story about this kind of thing. She had been performing transposon mutagenesis (while she was a postdoc with Fred Ausubel at Harvard) on Rhizobium meliloti (now Sinorhizobium meliloti). Sharon isolated a transposon mutant incapable of forming root nodules on alfalfa.

A Harvard professor asked her how she knew that the mutant was Rhizobium. Sharon replied that it has the appropriate antibiotic resistance markers. The questioner pointed out that the *definition* of Rhizobium meliloti included the ability to nodulate alfalfa roots. The mutant did not. So was it still Rhizobium meliloti??!!

That story was the first time I became uneasy with the species concept among prokaryotes. It wasn't the last!

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