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Paul said: "Another possibility is that YFB creates waste products which don't diffuse far from the colony and thus prevent the colony from growing very large." I'll buy that over nutrient depletion or agar drying out, at least as long as YFB isn't a swarmer.

As for the corallary, I'd think that if you streaked away radially from the edge, you should get growth again. These cells would have been the most actively growing before the growth inhibition due to the buildup of waste products, so they should resume growth right away. Besides, they taught us to inoculate from the edge of the colony, so that must be the right answer. :)

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