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kevin young

Regarding your “Rafting through Time”…

The “membrane domain” question (or lipid raft question) is interesting. We also published, long ago, information that suggested that there were more than one “domain” associated with bacterial membranes (see three references below). Our observations fell short of proving anything like lipid rafts, but did suggest that distinct protein-membrane associations existed. Common preparation techniques obscured some of these. I notice that we were evidently not aware of the Green and Schaechter paper, either, at that time, because we didn’t reference it (sorry!).

Jacoby, G. H., and K. D. Young. 1988. Unequal distribution of penicillin-binding proteins among inner membrane vesicles ofEscherichia coli. J Bacteriol 170:3660-7.

Leidenix, M. J., G. H. Jacoby, T. A. Henderson, and K. D. Young. 1989. Separation of Escherichia coli penicillin-binding proteins into different membrane vesicles by agarose electrophoresis and sizing chromatography. J Bacteriol 171:5680-6.

Jacoby, G. H., and K. D. Young. 1990. Heterogeneity among membrane vesicles of Escherichia coli: effects of production and fractionation techniques. Anal Biochem 184:48-54.

Kevin Young

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