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Kevin D Young

Hi Elio (and Joan),

Indeed the GFP fusion question was one we worried about. In the paper, we worked very hard to be sure that GFP was not the reason the protein was going to the poles. One of the best things thing we did (I think) was compete the polar foci away from the poles by expressing the wild type proteins without the GFP tag. Not only was each protein released from the poles in the presence of the wild type version, but each protein was competed away only by its own wild type, not by expressing the other protein (so the effect was very specific). Also, the original polar localization was determined by isolating polar membrane vesicles where only the wild type proteins were being made.

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