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Fernando Sorroche

In one of the comments it was suggested to do a new experiment making the metabolic requirements non-overlapping because in the original experiment the initial selection and the final selection would have the same enzymes (given that there is glucose in both rich and minimal medium). If I understood correctly, I asume Ole did not intended to specifically detect mutants in glucose metabolism with that experimental design, so I don´t get the point of that part of the comment. The initial and final selection are not just made on the base of glucose metabolism. There are many metabolic reactions that are different in both situations, (for example aminoacid anabolic reactions in the minimal medium that would allow to detect aminoacid auxotrophies)

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